Anti-Tumor Effect and Its Mechanism of Vaccaria segetalis on Mouse Inoculated H22 Solid Carcinoma

摘要

AIM Vaccaria segetalis was reported anti-tumor action in vitro. In this study, the effect and mechanism of inhibiting tumor growth and improving the quality of life of Vaccaria segetalis on mouse inoculated H22 solid carcinoma were investigated. METHODS The solid carcinoma model of mouse was made by H22 cells subcutaneous injection and they were divided into four groups including model control group, 1mg/kg treatment dose, 2.5mg/kg treatment dose and 5mg/kg treatment dose groups of Vaccaria segetalis.After ig treatment for 10 days,the mice were killed,and the inhibited rate of tumor growing was calculated. The pathological morhpologic change of tumor was observed by HE staining.The apoptosis number of tumor was observed by TUNEL method. The expression of CD31 of tumor endothelial cells was measured by immunohistochemical method.RESULTS Vaccaria segetalis can improve the quality of life on mice invased by tumor, inhibite the growth of tumor.The effect of Vaccaria segetalis on the tumor morphology showed nuclear shrinkage, necrosis, and inflammatory cell infiltration on the periphery, etc. And it inhibited the expression of CD31, induced apoptosis of tumor cells and vascular endothelial cells.CONCLUSION Vaccaria segetalis can improve the the quality of life on mice invased by tumor, and inhibit the growth of tumor. The mechanisms were likely due to inhibiting the angiogenesis, and inducing the apoptosis of tumor cells and vascular endothelial cells.