Effects of Qinglongyi Polysaccharide on complex biochemisty function with the core of Band 3 on Erythrocyte

摘要

Objective:To explore the effects of Qing long yi polysaccharide on biochemical functions of band 3 core complex in S180 mouse erythrocyte membrane.Methods:We use SDS-PAGE method and G-250 dye to study the effect of qing long yi polysaccharide on band 3 content of S180 mouse erythrocyte,use LSCM to measure the concentration of H+ in erythrocyte of S180 mice,use fluorescence spectrophotometry to determine membrane fluorescence polarization and microviscosity,use spectrophotometer to measure sialic acid content on erythrocyte membrane,ATPase test kits to determine the activities of Na+,K+-ATPase and Ca2+,Mg2+-ATPase,use FCM to measure membrane potential.Results:It showed medium(50mg/kg) and high-dosage (100mg/kg) of Qing long yi polysaccharide increased band 3 content.The result showed medium-dosage (50mg/kg) of Qing long yi polysaccharide signally increased band 3 function on CI-transportation,decreased the concentration of H+,decreased the concentration of Ca2+,increased membrane fluidity,increased sialic acid content,enhanced the activities of Na+,K+-ATPase and Ca2+,Mg2+-ATPase,heightened membrane potential.Conclusion:Qing long yi polysaccharide can improve biochemical functions of band 3 core complex in S180 mouse erythrocyte membrane,Which enhance the immunity function of erythrocytes,it may be one of the most important antitumor mechanisms.