A novel method for detecting and configuring bacteria using a micro-scale pore is presented. The method distinguishes between different species of bacteria by measuring the ionic current blockage (resistive pulse) as bacteria electrophoretically translocate the micro-pore. Both wild-type flagellated (HCB 33) and non-flagellated Escherichia coli (HCB 5), and Polystyrene microbeads were used in this study to demonstrate the efficacy of this method. High resolution electrical signal readout enabled discrimination of the orientation of both non-flagellated and peritrichously flagellated bacteria as they move through the solid-state pore.
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