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MICROSYSTEM SURVEYING THREE DIMENSIONAL (3D) CULTURE AND EMBRYO DEVELOPMENT

机译:微系统研究三维(3D)文化和胚胎发育

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We have developed microdevices to handle, manipulate, culture and characterize cells and embryos. In the present study, the polarities of the human mammary epithelial cell line (S- 1) and its malignant cell line (T4 - 2) were controlled using a cellular array chip. The cells were embedded with 20 nL of reconstituted basement membrane matrix (Matrigel) on a pyramidal-shaped silicon microstructure. The proliferation behavior, the respiration activity, and the gene expression analysis have been carried out to characterize cellular functions on chip and discussed from the view point of the cellular polarity.
机译:我们已经开发出微设备来处理,操纵,培养和表征细胞和胚胎。在本研究中,使用细胞阵列芯片控制人乳腺上皮细胞系(S-1)及其恶性细胞系(T4-2)的极性。细胞在金字塔形的硅微结构上嵌入了20 nL的重组基底膜基质(Matrigel)。已经进行了增殖行为,呼吸活性和基因表达分析,以表征芯片上的细胞功能,并从细胞极性的角度进行了讨论。

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