Survival of SARS Virus in Vitro

摘要

Objective Evaluate the stability and infectious of SARS virus in patient's excrements and contaminated objects in vitro. Method Two strains of SARS virus were used in this study, one was BJ - 01 isolated from the lung tissue of one patient in Beijing, The other was GZF isolated from sputum of one patient in Guangzhou, The virus were cultured and propagated with Vero - E6 cell, the medium was DMEM supplemented with 10% fetal bovine serum. (1) Adding SARS virus (10~6TCID_(50)) to the specimen of faeces, urine, sputum, and plasma collected from healthy individuals, depositing in the cleaning area of BSL - 31ab, sampling 0. 5ml after definite time points, centrifuging, filtering and titering in Vero - E6 cell, observing CPE. (2) Smearing 10~6TCID_(50) SARS virus on the surface of sterilized steel slice, glass slice, plastics slice, paper slice, fabric slice and wood slice, adding the virus to sterilized water and soil. Rinsing and infecting Vero - E6 cell after definite time points. (3) The experiments were repeated three times. Result (1) SARS virus survival at least 3 days in the analogue sputum of SARS patient. The virus is stable at least 4 days in the analogue faeces of SARS patients. The virus keep high liter as long as 10 days in the analogue urine and plasma of SARS patients. (2) SARS virus keep infectious at least 48 - 72 hr in the analogue contaminated steel slice, glass slice and plastics slice, The infectious liter reduced about 5log within 48 hours. The virus can survival 4 - 6hr in the analogue conlaminal-ed paper slice, fabric slice and wood slice, 8hr in contaminated soil, 10 days in contaminated water. (3) The survival lime of BJ - 01 slrain and GZF strain in those specimens mentioned above were nol significantly difference. Conclusion SARS virus can survival long lime after discharge outside human body. It might be transmitted by faeces - oral mode and by contaminated environment. It is very important to sterilize patient' s excrement and contaminated objects effectively.