Fast laser-scanning microscopy with single- and multi-photon excitation for functional imaging of living nerve cells

摘要

Functional neuro-imaging is often limited by the spatio-temporal resolution of available imaging systems. The goal of this project is fast multi-site imaging of nerve cells in intact mammalian brain tissue. We will review fundamentals of fast laser-scanning microscopy employing acousto-optic beam steering and show examples of neuro-imaging applications. Specifically, we will discuss the significance and technical challenges of extending this imaging approach from single- to multi-photon excitation. We will present the current development status of a new imaging system implementing acousto-optical scanning of ultra-fast laser pulses.