APPARATUS FOR REAL-TIME THREE-DIMENSIONAL LASER SCANNING MICROSCOPY, WITH DETECTION OF SINGLE- AND MULTI-PHOTON FLUORESCENCE AND OF HIGHER ORDER HARMONICS

摘要

Apparatus for real-time three-dimensional laser scanning microscopy, where single-photon fluorescence light, multi-photon fluorescence light, and higher order harmonics generated in the sample are detected. The excitation light is focused into the sample in a three-dimensional matrix of focal points. Real-time three- dimensional image acquisition is obtained by fast scanning in the xy plane only.