Detection of Enteric Viruses in Archived ICR Sample Concentrates Using an Integrated Cell Culture-Nested PCR Technique.

摘要

The preamble of the Information Collection Rule (ICR) referenced the goal of archiving virussamples for future study. From samples submitted to our laboratory during the ICR, AnalyticalServices, Inc. (ASI) and University of New Hampshire (UNH) created a repository of sampleconcentrates that were representative of a broad geographic area and were consistent with respectto methods of sample collection, handling and storage. The objective of this study was toexamine viral occurrence in archived source water sample concentrates using a sophisticated,sensitive molecular technique and to compare these results to the respective data obtained fromthe Total Cultivatable Virus Assay (TCVA) used in the ICR.