MODULATION OF INDIRECT NEUROTRAUMA BY LOW LEVEL LASER STIMULATION IN RELATION WITH APPLIED WAVELENGTH

摘要

Previous studies show that low level laser (LLL) irradiation can modulate biochemical processes taking place in inflammatory and ischaemic conditions, in relation with applied wavelength of LLL. We undertook our study to investigate whether transcranial application of LLL beams could modify biochemical disturbances of brainstem induced by moderate pulmonary blast injury (PBI) in relation with applied wavelength of LLL. A total of 42 adult rabbits were used in this study, randomly assigned in 6 groups: 1. Control 2. Intact animals treated with LLL in wavelength of 660nm 3. Intact animals treated with LLL in wavelength of 904nm 4. PBI animals LD_(50) 5. PBI animals were consequent treated with 660nm LLL stimulation 6. PBI animals were consequent treated with 904nm LLL. Blast wave with nominal peak pressure of 304 kPa was generated in laboratory conditions, using air driven shock tube. Using Irradia Mid 904 multiprobe and 660 nm singleprobe at dose of 0,1J/cm2 performs laser stimulation. The lipid peroxidation activity and activity of enzymes of antioxidative defense were measured. Both applied wavelength of LLL significant increased the lipid peroxidation activity. 904nm of LLL significant increase activity of enzymes of antioxidative defense in conditions of posttraumatic decreases of these enzymatic systems, induced by PBI.