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Video-rate spectral imaging of fluorescence phonomena

机译:荧光声子的视频速率光谱成像

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Abstract: We describe fluorescence spectral-imaging results with the microscope computed-tomography imaging spectrometer ($mu@CTIS). This imaging spectrometer is capable of recording spatial and spectral data simultaneously. Consequently, the $mu@CTIS can be used to image dynamic phenomena involving multiple, spectrally overlapping fluorescence probes. The result presented in this paper consists of proof-of-concept imaging result using two static targets. The first is composed of 6-$mu@m fluorescing microspheres and the second consists of rat sinusoid epithelial cells loaded with 0.5-$mu@m fluorescing microspheres. Image data were collected in integration times of 16 msec, comparable to video frame rate integration times. The emission spectra were sampled at 10-nm intervals between 420 nm and 710 nm. The smallest spatial sampling interval presented in this paper is 1.7 $mu@m. !19
机译:摘要:我们用显微镜计算机断层成像成像光谱仪($ mu @ CTIS)描述了荧光光谱成像结果。该成像光谱仪能够同时记录空间和光谱数据。因此,$ mu @ CTIS可用于对涉及多个光谱重叠的荧光探针的动态现象进行成像。本文提出的结果包括使用两个静态目标的概念验证成像结果。第一个由6-μm荧光微球组成,第二个由载有0.5-μm荧光微球的大鼠正弦上皮细胞组成。图像数据的采集时间为16毫秒,与视频帧速率的积分时间相当。发射光谱以420 nm和710 nm之间的10 nm间隔采样。本文提出的最小空间采样间隔为1.7μm@m。 !19

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