Abstract: In this paper, a simple chemical method of urease immobilization on silicon nitride surface is described. As a basic structure to construct urea-biosensor, a pH-sensitive Si$-3$/N$-4$/-gate ISFET was used. The developed method of chemical immobilization of urease is based on Schiff's base formation. The developed EnFET type urea biosensor are characterized by the following parameters: (1) maximum analytical signal: 120 $DIV 140 mV in 10 mM phosphate buffer solution, (2) linear range of the $Delta@U$-gs$/ $EQ f(logC$-urea$/): pC$-urea$/(2 $DIV 3.5) in 10 mM phosphate buffer, (3) response time: 80 s and (4) lifetime: 35 days with the stable analytical signal then after 52 days this signal decreased by at least 40%. The influence of the concentration as well as the pH of the buffer solution on EnFET response were investigated.!16
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机译：摘要：本文描述了一种简单的化学方法将尿素酶固定在氮化硅表面。作为构造尿素生物传感器的基本结构，使用了对pH敏感的Si $ -3 $ / N $ -4 $ /门ISFET。已开发的化学固定脲酶的方法是基于席夫碱的形成。已开发的EnFET型尿素生物传感器的特征在于以下参数：（1）最大分析信号：在10 mM磷酸盐缓冲溶液中为120 $ DIV 140 mV，（2）$ Delta @ U $ -gs $ / $ EQ的线性范围f（logC $ -urea $ /）：在10 mM磷酸盐缓冲液中的pC $ -urea $ /（2 DIV 3.5），（3）响应时间：80 s和（4）寿命：35天，然后产生稳定的分析信号52天后，该信号至少下降了40％。研究了缓冲溶液的浓度以及pH值对EnFET响应的影响！16
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