ARTIFICIAL CELLS CONTAINING GENETICALLY ENGINEERED E. COLI DH5 CELLS FOR UREA AND AMMONIA REMOVAL IN KIDNEY AND LIVER FAILURE

摘要

This paper describes a novel method of urea and ammonia removal using microencapsulated genetically engineered E. coli DH5 cells. In vitro, 100 mg of bacterial cells encapsulated in alginate-polylysine-alginate (APA) membrane, in their log phase state of growth, can lower 87.89 ± 2.25 % of the plasma urea within 20 minutes and 99.99 % in 30 minutes. The same amount of encapsulated bacteria is also capable of lowering ammonia from 975.14 ± 70.15 μM/L to 81.151 ± 7.37 μM/L in 30 minutes. This new approach is significantly more efficient than the presently available methods of urea and ammonia removal.