AptaBlocks: Accelerating the Design of RNA-Based Drug Delivery Systems

摘要

Synthetic RNA molecules are increasingly used to alter cellular functions [1-4]. These successful applications indicate that RNA-based therapeutics might be able to target currently undruggable genes [5, 6]. However, to achieve this promise, an effective method for delivering therapeutic RNAs into specific cells is required. Recently, RNA aptamers emerged as promising delivery agents due to their ability of binding specific cell receptors [7, 8]. Crucially, these aptamers can frequently be internalized into the cells expressing these receptors on their surfaces. This property is leveraged in aptamer based drug delivery systems by combining such receptor-specific aptamers with a therapeutic "cargo" such that the aptamer facilitates the internalization of the cargo into the cell [9-11]. The advancement of this technology however is contingent on an efficient method to produce stable molecular complexes that include specific aptamers and cargoes. A recently proposed experimental procedure for obtaining such complexes relies on conjugating the aptamer and the cargo with complementary RNA strands so that when such modified molecules are incubated together, the complementary RNA strands hybridize to form a double-stranded "sticky bridge" connecting the aptamer with its cargo [12, 13]. However, designing appropriate sticky bridge sequences guaranteeing the formation and stability of the complex while simultaneously not interfering with the aptamer or the cargo as well as not causing spurious aggregation of the molecules during incubation has proven highly challenging.