New method of SERS-active gold surfaces fabrication for bacterial cells Raman analysis

摘要

Paper performs results of SERS-active surfaces fabrication for Raman bacterial cells analysis. Based on FDTD simulation, the synthesis of colloidal gold nanoparticles (NPs) with the size range of 10 - 100 nm has been performed by the following methods: a) femtosecond laser ablation of a plate in a liquid; b) chemical reduction from tetrachloroauric acid trihydrate (HAuCl_4). Optimal sizes and shapes of the particles with a maximum of plasmon absorption in the range 500 - 800 nm have been determined by numerical simulation. For NPs deposited on quartz glass with rodamine 6G (R6G) and E. Coli bacterial cells, SERS solutions have been tested.