A bioengineered 3D printed calciumalkaliorthophosphate-based bone graft facilitates repair of critical-size segmental discontinuity bone defects in vivo

摘要

Histomorphometric, immunohistochemical and angio-μCT analyses demonstrated that implantation of 3D printed CAOP scaffolds, pre-colonized in vitro with homogenously distributed osteoblasts and mineralizing bone matrix, in combination with using the microvascular AVB technique during surgery is an excellent tissue engineering approach for achieving excellent bone formation and repair of critical-size segmental discontinuity defects in vivo.