Enhancing the Stability and Potency of the Adjuvant Poly(I:C) through Nanoparticle Encapsulation

摘要

I synthesized, characterized, and screened a combinatorial library of poly(I:C)-loaded PBAE NPs. Many polymer NPs enhanced the potency of poly(I:C) in vivo and NP 5 led to an unprecedented 13X increase in activation and did not cause systemic toxicity. I selected promising NPs and tested their ability to enhance the immune response to ova antigen in vivo. I found that NP 5 greatly enhanced the humoral immune response (increased antibody concentration and affinity) to ova. Additionally, vaccination with NPs of varying polymer chemistry altered the IgG1:IgG2c ratio, suggesting we can tune polymer chemistry to skew the immune response (Th1 vs. Th2).