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Identification of Flavonoid from Leaves of Gedi (Abelmoschus manihot L.) and Its Antioxidant Activity

机译:鉴定GEDI(Abelmoschus Manihot L.)叶片的黄酮类化合物及其抗氧化活性

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A flavonoid compound had been isolated from Abelmoschus manihotL. leaves. The leaves extract was obtained by maceration in methanol to obtain n-hexane-acetone-methanol sequential extracts. Further separation was done by flash chromatography using silica gel G - 60 as a stationary phase. The column was eluted subsequently using several mixture solvents including ethyl acetate, ethyl acetate/methanol (3:1,v/v,), ethyl acetate/methanol (1:1, v/v), ethyl acetate/methanol (1:3) and methanol at 2 mL/min flow rate. At the end of this fractionation, 112 fractions of 10 mL each was collected. Using bioassay-guided fractionation, seven isolates from fraction four were obtained. The isolate five showed the highest antioxidant activity in the 2,2-diphenyl-1-picrylhydarzyl (DPPH) radical scavenging test and then it was chosen for structure elucidation. The structure of the compound was determined based on spectroscopic data of UV, IR, ~1H-NMR, and ~(13)C-NMR. The isolated compound was proposed as 3,3',5' trihydroxy-4' methoxy flavone. It exhibited antioxidant activity, with IC_(50) of 727.53 μg/mL.
机译:从阿伯马斯科汉胆管分离出一种类黄酮化合物。树叶。通过甲醇中的钙获得叶子提取物,得到正己烷 - 丙酮 - 甲醇顺序提取物。通过使用硅胶G-60作为固定相,通过快速色谱法进行进一步分离。随后使用几种混合物溶剂洗脱,包括乙酸乙酯,乙酸乙酯/甲醇(3:1,V / V),乙酸乙酯/甲醇(1:1,v / v),乙酸乙酯/甲醇(1:3 )和甲醇以2mL / min流速。在该分馏末端,收集每次112分级分。使用生物测定引导分馏,获得来自级分4的7个分离物。分离物五显示出2,2-二苯基-1-吡啶甲丁丁基(DPPH)自由基清除试验中的最高抗氧化活性,然后选择其用于结构阐明。基于UV,IR,〜1H-NMR和〜(13)C-NMR的光谱数据测定化合物的结构。将分离的化合物提出为3,3',5'Tihydroxy-4'甲氧基黄酮。它表现出抗氧化活性,IC_(50)为727.53μg/ ml。

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