Ultra scale-down mimics for perfusion culture: Experimental study for rapid biopharmaceutical process development

摘要

With the industry considering the implementation of end to end continuous bioprocesses, there is a need for the development of scale-down tools to provide the same consistency, reliability and throughput as the scale-down tools already available for traditional fed-batch processing. This work aims to develop a perfusion scale-down system capable of reproducing the specific characteristics of the perfusion culture process, namely cell retention capabilities, the ability to support high cell densities and to operate for extended periods compared to fed-batch cultures. Cell culture in microwell plates in fed-batch mode is well defined and is in widespread use; however to the best of our knowledge this represents the first attempt at the development of quasi-perfusion cell culture at this scale.