Application of single pass TFF to enable intensified and continuous biological manufacturing

摘要

Intensified polishing: A new application which links SPTFF using Pellicon cassettes and Eshmuno? Q anion exchange resin 1. Improve isotherm binding conditions by pre-concentrating feed. 2. 4x improvement in Eshmuno? Q resin mass loading while maintaining HCP and viral clearance. Ideal for next generation continuous process applications 3. Concentrate mAb product without recirculation tanks. 4. Reduce volume requirements for pH/conductivity adjustment. 5. Reduce process tank volumes. Improve process economics 6. Boost Eshmuno? Q resin productivity: increase mass load, decrease cyde time. 7. Low SPTFF cost provides significant savings in resin, buffer, and sterile filtration. 8. Reductions in tank size and pump requirements improve facility economics.