EVALUATION OF PSEUDO-PERFUSION FEEDING STRATEGIES FOR mAB PRODUCTION USING A CHO CELL LINE ADAPTED TO CONCENTRATED FEED MEDIA

摘要

Vitoria Sao Jose, Federal University of Rio de Janeiro (UFRJ), COPPE, Cell Culture Engineering Laboratory Leda R. Castilho, Federal University of Rio de Janeiro (UFRJ), COPPE, Cell Culture Engineering Laboratory Perfusion has been intensively studied for biopharmaceuticals production due to the high volumetric productivities over long periods in operation. However, high dilution rates (D) used to feed basal media for therapeutic protein production imply in the generation of large harvest volumes, causing a dilution of the product of interest (POI) and increasing purification costs. Previous pseudo-perfusion studies of our group have indicated the possibility of getting a more concentrated POI (a monoclonal antibody produced by a CHO cell line) in the harvest stream using as perfusion feed a concentrated medium (as opposed to basal medium) at lower dilution rates. However, a decrease in cell viability and volumetric productivity (Pv) due to the higher osmolality related to the concentrated media were observed over cultivation time.