Electroconductive hydrogel: Hyaluronic acid-based hydrogel decorated with electroconductive nanomaterials for human neural stem cell engineering

摘要

In summary, this study demonstrates catechol-mediated dispersion and incorporation of CNTs and PPy into HA-CA hydrogel to improve electrochemical properties of the hydrogel. We showed that the conductive 3D environment provided by the CNT/PPy-containing HA-CA hydrogels was beneficial for neuronal differentiation of NSCs by showing that calcium channel expression was upregulated, depolarization was activated, and intracellular calcium influx was increased in human stem cells that were stimulated with electrical signals, and these outcomes are indicative of stem cell neurogenesis. Thus, 3D electroconductive HA hydrogel can provide a promising culture platform and tissue-engineering scaffold for stem cell therapy to treat neurodegenerative diseases.