These preliminary studies demonstrate that we can surface-functionalize iron oxide NPs with PEG and LDH B peptide. NP characterization confirmed the desired size, stability, and surface modifications. Our NP formulation would serve as the first prophylactic against MAR autism by clearing MAR Abs resulting in normal fetal development. To ensure maximal specific MAR Ab binding and repulsion of non-specific Abs, we will engineer our NP formulation by manipulating parameters such as PEG density and molecular weight. Our future studies will include (a) in vitro biological characterization of epitope-functionalized NPs (e.g. cellular toxicity, uptake, and epitope specificity of the antigen-conjugated NPs), (b) in vivo pharmacokinetic study in pregnant mice, and (c) efficacy of the NP system in MAR autism mice model by assessing behavioral changes in the offspring.
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