The Influence of Mechanical Rubbing on the Dissolution of Blood Clots

摘要

Mechanical rubbing of blood clots is a potential minimally-invasive method for clearing clogged blood vessels. In this work, we investigate the influence of the interaction of the tip of a helical robot with blood clots. This interaction enables the dissolution of the blood clot and the release of the entrapped red blood cells and platelets from its three-dimensional fibrin fiber network. We analyze the pre- and post-conditions of the blood clots following 40 minutes of mechanical rubbing, under the influence of a rotating magnetic field in the frequency range of 20 Hz to 45 Hz. Our measurements show that the weight of the blood clots is decreased by 22.5 ± 11.1% at frequency of 25 Hz. We also validate the influence of mechanical rubbing using cell count and spectrophotometric analysis on phosphate buffered saline samples past the robot and the clot. The maximum cell count is measured as 654 ± 108 × 10⁴ cells/ml and 54 ± 12 × 10⁴ cells/ml, whereas the absorbance is measured as 4.35 × 10^-6 mol and 1.05 × 10^-6 mol under the influence of mechanical rubbing and without mechanical rubbing, respectively.