High-Efficiency Separation and Purification of Taq DNA Polymerase

摘要

The thermostable Taq DNA polymerase was firstly isolated by Ms. Qian Jiayun from the YT-1 strain of the aquatic thermophilic bacteria (Thermus aquatics) [1-3]. Its high specificity, yield and sensitivity make it widely used in PCR [4, 5] and other related techniques for many years. Taq polymerase has a half-life of 9 min at 97.5 °C and its optimum temperature is 75-80 °C. It can replicate a DNA fragment of 1000 bp within 10 s at 72 °C. Its higher enzyme activity is temperature dependent. At low temperature, the enzyme activity of replicating DNA was significantly lower comparing to that at higher temperature. However, its activity on DNA synthesis is also quickly reduced when temperature is higher than 90 °C.