Oxidized-Alginate Microbead Encapsulated Osteoclasts as a Cell-based Therapy to Reverse Vascular #Calcification

摘要

Conclusion: We were able to properly form microbeads with the 1% oxidized and non-oxidized alginate solutions, but no beads materialized from the 2% oxidized alginate. This suggests that the alginate is strongly sensitive to oxidation via sodium periodate. A degradation study (not shown) revealed the 1% oxidized alginate microbeads to be . the preferred scaffold choice because of the fast degradation exhibited. The 1% oxidized alginate microbeads significantly degraded throughout the 72-hour study. The non-oxidized microbeads however, showed virtually no significant degradation under identical conditions. A fast-releasing delivery vehicle is preferred because it will help alleviate problems that could occur during in vivo applications regarding cell viability and injection attachment stability at target locations. Taken together, the results suggest that the oxidized alginate microbeads could potentially serve as delivery vehicle for cell-based therapies. We will continue to optimize our alginate oxidation protocol to adjust microbead degradability as needed. Our next steps will involve cell encapsulations with the oxidized alginate microbeads towards testing proliferation and viability in normal culture media, and ultimately in vivo applications of the osteoclast-based therapy.