Characterization of biomolecules using an aptamer-based graphene nanosensor

摘要

We present binding properties of biomolecules and their dependence on ionic strength and temperature using a microfluidic aptameric graphene field-effect transistor (FET) nanosensor. Aptamer-immobilized graphene is used to recognize target molecules, and the resulting changes in graphene conductance is measured to study the binding kinetics of biomolecules. Binding properties of aptamer-protein interactions at different ionic strength and temperature are investigated, yielding insight into the pharmacologic basis of biomolecular recognition.