Detection of methicillin-resistant Staphylococcus aureus of poultry and human sources

摘要

Staphylococcus aureus(S. aureus ) is a common gram positive anaerobic facultative pathogen of human and birds; and mostly resides on skin, wounds, mucous membranes, alimentary & urogenital tracts, soft tissue infections. Resistance in S. aureus against antibiotics has been increasingly reported though depending on the clonal lineage. Among the resistant strains methicillin resistant S. aureus (MRSA) is a major threat to the community as its genome is more proneto evolution. The emergence of MRSA is distressing in community that embodies a model for emergence of this uncontrollable super bugs. The molecular based detection and characterization of MRSA has been unstudied in the local population of human and poultry. In the present study50 isolates (n= 42 were recovered from poultry external naries, respiratory tract and feathers) and (n= 08 were recovered from naries and hands of poultry workers) were used after biochemical and bacteriological identifications. MecA gene (527 bp)was amplified for the detection of MRSA followed by and sequencing using specially designed primers. The obtained sequences were compared with reported data and phylogenetic analysis was done. Comparative analysis indicated 2 polymorphic sites in the local isolates. Phylogenetic analysis showed that local poultry isolates were mono-phyletically claded with S. aureus MRSA and shared the same clade with human MRSA isolates. This indicated that this organism has a zoonotic potential. This is the first report of detection and sequencing of MRSA isolates recovered from poultry and human samples.