Fabrication of poly-(ε-caprolactone):poly-(octanoic acid 2-thiophen-3-yl-ethyl ester) fibers by electrospinning for cell proliferation

摘要

Introduction: Muscle tissue regeneration, when there is a great loss of muscle, continues to be a challenge tor tissue engineering. In this field, scaffolds that have similar physical properties to the native cellular matrix are promising; hence substrates made of fibers are important in the regeneration of muscle tissue.Biological processes such as cell migration and signaling are complex because they require dynamic interactions between cells and the native extracellular matrix. Obtaining this biocomplexity with synthetic and/or natural polymers that have been studied remains a challenge and is undoubtedly important for improving the potential of synthetic scaffolds point). Considering the above, we prepared polymeric scaffolds by electrospinning with biodegradable, electroactive and biocompatible properties that can provide both mechanical support as well as electro activity to promote cell proliferation and differentiation. Materials and Methods:The composites of poly-(e-caprolactone):poly-(octanoic acid 2-thiophen-3-yl-ethyl ester)(PCL:poly-(OTE)) in 90:10 ratio respectively obtained from the mixture of PCL of 80 kDa of Mw and (poly-(OTE) synthesized in our laboratory. The fibers were produced by electrospinning using a rotary collector covered with a copper foil. The applied voltage was 22 kV, the flow rate of the solution was 0.4 mL/h, the gap between the needle and collector was 15 cm and the rotational speed was 2200 rpm. Biocompatibility tests were performed using a mouse myoblast cell line C2C12 cultured on polymer fibers. For the observation of nuclei and cell morphology, samples were fixed and then stained with hematoxylin-eosin. Images were obtained using an optical microscope equipped with a camera MEM1300. Results and Discussion: The synthesized poly-(OTE) was characterized by Fourier transform infrared (FTIR), absorption bands of the corresponding compound were evidenced in: 2932-2900 cm~(-1) aliphatic (C-H), 1748 cm~(-1) carbonyl group (C=O) and a new band at approximately 840 cm~(-1) for trisubstituted thiophene ring in positions 2,3 and 5, at 1644 cm~(-1) corresponding to the signal due to polyconjugation. These signals are consistent with monomer polymerization. To obtain PCLpoly-(OTE) fibers the collector's rotational speed was evaluated. It was noted that the increase from 500 to 2200 rpm the fibers went from having no orientation to being highly aligned, as evidenced by Figure 1. Additionally, PCL fibers were prepared at 15% w/v, and used as a control in cellular assays. In general, it was observed that, compared to PCL fibers, the presence of poly-(OTE) in the mixture reduced fiber collection time. In biological experiments it is generally observed that the scaffolds constructed by polymer fibers containing poly-(OTE) affect how cells adhere, preliminarily showing that cells align in the general direction of the fibers. Figure 1. Fibers PCLpoly-(OTE) obtained by electrospinning, using a rotary collector at 2200 rpm. Conclusion:The results suggest that use scaffolds made of blends of biodegradable and conductive polymers that possess similar architectures to those presented by muscle fibers shows promising preliminary results for myogenesis.