Choline phosphate functionalized cellulose membrane developed as potential hemostasis dressing based on a unique bioadhesion mechanism

摘要

A choline phosphate functionalized biocompatible cellulose membrane that can efficiently arrest human red blood cells was developed to have potential application in wound dressing. The bioadhesion is based on the unique multivalent electrostatic interaction between the head groups of phosphatidyl choline based lipids on the cell membrane and its inverse orientation but virtually identical structure, choline phosphate, coupled to the cellulose membrane. For functionalization, the cellulose membrane was decorated with the polymer brushes bearing multiple choline phosphate groups via surface-initiator atom transfer radical polymerization followed by the click chemistry. The molecular weight and the grafting density of polymer brushes grafted from the cellulose membrane surface were thoroughly evaluated by force-distance measurements using atomic force microscopy. The dependence on the number density of choline phosphate groups and the grafting density of human red blood cell binding to the cellulose membrane surface is reported.