Conductive Polypyrrole-polycaprolactone Scaffolds for Electrophysiological Maturation of Cardiomyocytes

摘要

This study demonstrated that conductive PPy-PCL films effectively support cardiomyocyte culture. PPy-PCL substrates promoted cellular attachment at comparable densities and cell areas relative to NaOH-treated PCL films and without a change in cellular viability. Cx43 gene expression was similar for HL-1 cardiomyocytes grown on PPy-PCL and PCL films. However, localization of Cx43 protein differed. Additionally, HL-1 cell sheets grown on conductive PPy-PCL films supported significantly faster calcium wave propagation and significantly lower calcium transient durations relative to HL-1 cell sheets grown on control PCL films. Therefore, PPy-PCL may provide effective cardiogenic tissue engineering scaffold.