Primary studies on the chemical constituents of Jinkuishenqi Pills metabolized in vivo by LC-MS

摘要

To identify the chemical constituents in Jinkuishenqi pills metabolized in vivo. Analysis and comparison were carried out by LC- ESI-MS among rats' serum samples obtained after oral administration of different ingredient preparations such as Jinkuishenqi pills, Jinkuishenqi Pills without one of ingredients and single ingredient crude drug and control serum. The compounds absorbed into blood, their original crude drugs and their metabolites were identified. Seventeen compounds were detected after oral administration of Jinkuishenqi Pills, six of that are metabolites and others are the original form of the compounds contained in Jinkuishenqi pills. Identified and presumed 12 constituents absorbed into blood by LC- ESI-MS technique. Among the total, the prime contents are galic acid, 5-HMFA, morroniside, loganin, Oxypaeoniflorin, paeoniflorinhandhpaeonolh The metabolized contents Gare, 4-dihydroxyacetophenone, 4-methoxylacetophenone-2-O-glycuronate, h2, 4-dihydroxyacetophenone-S-O-sulphate, 2-dihydroxy-4-methoxylacetophenone-S-O-sulphate. The compounds absorbed into blood and its metabolites are capable of being the effective constituents, the results provide a basic data for the further studies on effective constituents and the effecting mechanism of Jinkuishenqi pills.