Improvement of Electrotransformation Efficiency by Lysozyme Treatment in Cronobacter sakazakii

摘要

Many Cronobacter sakazakii strains were poorly transformable according to our previous study. In order to improve the efficiency of electro-transformation, lysozyme treatment was introduced in preparation of the competent cells. After optimization of the parameters critical for the application of electrotransformation, including lysozyme concentration, lysozyme disposing time, electric field strength, and the time constant, efficiency of electrotransformation was raised from 7.2 × 10~1 to 1.16 × 10~3 transformants per microgram DNA. To our knowledge, this is the first time that lysozyme was used to improve the transformation efficiency in Cronobacter.