Optimized numerical pharmacokinetics model for optical molecular probes based on diffusion coefficients in matrigel measured using fluorescence imaging

摘要

An original algorithm for measuring diffusion coefficients of optical molecular probes in matrigel from fluorescence data is introduced. The algorithm was developed in Fortran and linked to Graphic User Interface in LabVIEW software that also performs image acquisition and processing. The software models pharmacokinetics of optical molecular probes providing the best fit of experimental data. The paper offers an original way for estimating the diffusion path length through extracellular matrix (ECM) from the rate constants given by the model and from measured diffusion coefficients.