AUTOMATED ACTIN FILAMENT SEGMENTATION, TRACKING AND TIP ELONGATION MEASUREMENTS BASED ON OPEN ACTIVE CONTOUR MODELS

摘要

This paper presents an automated method for actin filament segmentation and tracking for measuring tip elongation rates in Total Internal Reflection Fluorescence Microscopy (TIRFM) images. The main contributions of the paper are: (i) we use a novel open active contour model for filament segmentation and tracking, which is fast and robust against noise; and (ii) different strategies are proposed to solve the filament intersection problem, which is shown to be the main difficulty in filament tracking. Application to experimental results demonstrated the robustness and effectiveness of this method.