We have successfully developed a protocol that allows us to prepare microspheres from RK-modified CS via w/o emulsion technique. While the size range is relatively large, the majority of microspheres fell between 30 and 90 μm diameter. The FTIR spectra demonstrated the imine bond peak appeared after modifying the chitosan with RK indicating that RK was bonded to the chitosan and not just absorbed to the chitosan polymer. The in vitro data demonstrates that the modified microspheres did not activate NO production at a higher level than the negative controls or unmodified chitosan groups. Further research is needed to explore whether the RKCS microspheres can inhibit an NO response from macrophages that are exposed to LPS, as well as degradation profiles.
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