Toll-like Receptor 2-mediated Inflammatory Response to Polymeric Biomaterials

摘要

These data shows that: (1) lysate-derived DAMPs competed with serum proteins for biomaterial surface interactions in vitro; (2) lysate-derived DAMPs adsorbed to polymer surfaces potently NF-kB transcription factors, to a greater extent than serum protein; (3) DAMP-adsorbed polymer surfaces activated NF-kB primarily through TLR2; and (4) exposure of RAW-Blue macrophages to DAMP-adsorbed surfaces strongly induced the production of pro-inflammatory cytokines IL-6 and TNF-a in a TLR2-dependent manner. These results suggest that DAMPs, in their adsorbed conformation, are capable of inducing a potent pro-inflammatory response in macrophages through TLRs, and that TLR signaling pathways should be investigated further as potential therapeutic targets for modulating host responses to implanted biomaterials.