Multi-stage lymphatic delivery system augments lymphoma immunotherapy

摘要

We were able to conjugate the TLR9 ligand CpG through our OND linkage to lymphatic-draining NP and demonstrate that CpG-OND-NP dramatically alter the abundance and type of lymphocytes within the treated LN, producing a large increase in total lymphocytes and a greater maturation of resident antigen presenting cells. We further demonstrated that this delivery strategy afforded a substantial reduction in LN tumor size compared to other CpG delivery strategies that eliminated LN lymphomas. These results suggest the utility of the multi-stage OND-NP approach for the delivery of immunotherapeutics to LN-resident cells for the treatment of LN tumors.