Riboflavin as a isible light photoinitiator for dynamic stiffening of cell-laden hydrogels

摘要

In summary, we have developed a dynamic hydrogel system using thiol-norbonene chemistry and riboflavin/visible light based in situ stiffening. Compared to other water soluble, visible light initiators (e.g., lithium acylphosphinate or LAP), riboflavin is naturally occurring, has higher molar absorbability at 400-500 nm-..The use of______ di-tyrosine crosslinking to stiffen_cell-laden hydrogels and does not produce heterogeneous crosslinks- and The the degree of stiffening could be readily controlled by adjusting tight intensity or tyrosine concentration. The system was highly cytocompatible for encapsulation of pancreatic cancer cells and the stiffening process did not induce cell deatheffectively affected cell fale in 3D. Future Current work is will be focused on optinizing the system and studying the effects of stiffened hydrogels on cells on at the molecular level.