Ultrathin Surface Coating of Thermoresponsive Block Copolymers for Fabricating Cell Sheets

摘要

Nano-scaled thin coating of PIPAAm-based polymers on commercial culture surfaces allowed cells to detach from the surfaces by reducing temperature. Chain length and mobility of grafted PIPAAm greatly affected both cell adhesion/proliferation and detachment. In addition, cell sheets with intact deposited extracellular matrix were able to be harvested from the smart surfaces.