Study on the miRNA-mediated regulatory network in the heart adjacent tissues of patients with tetralogy of Fallot

摘要

When Tetralogy of Fallot (TOF) disease occurs, the miRNA expression profile of the diseased tissues will change significantly, showing a disease-specific expression profile. However, the molecular mechanisms of its pathogenesis and development are still unclear. In this article, we selected the data set related to TOF disease and screened out the 149 differentially expressed (DE) miRNAs in the right ventricular outflow tract (RVOT) myocardial tissue and the 38 DE miRNAs expressed in the right ventricular (RV) myocardial tissue, as a result, it was found that two different miRNAs from adjacent tissues combined with target genes to construct a regulatory network. Research on the network and that these two networks meet the characteristics of a scale-free network. The functional analysis of the 64 target genes shared by the two adjacent regulatory networks showed that they were associated with TOF disease. 34 hub nodes were found in the RV myocardial tissue. 15 nodes were found in RVOT myocardial tissue. In the discussion section, we discussed the relationship between genes and drugs in the hub node, and analyzed the potential effects of three potential drugs on TOF disease by regulating the STAT3 gene. Therefore, this article analyzes the molecular level changes of the two types of myocardial tissues through miRNA-mediated regulatory networks, including the biological functions, signal transmission and network characteristics of adjacent areas and important nodules in TOF disease. Exploring the relationship between hub nodes and drug target.