Antifungal activity of endophyte cultures of Morus alba L. against phytopathogenic fungi

摘要

A total of 106 strains of endophytic fungi were isolated from mulberry tree.86 strains of the total isolates belonged to 18 genera.The dominant species were Fusarium spp.(22.6％) and Alternaria spp.(15.1％).The isolation frequency of endophytes was around 47,38 and 15％,respectively to roots,branches and leaves.About 54％ of the isolates could produce antifungal metabolites against at least one plant pathogenic fungi.Endophytic Aspergillus sp.A204,Colletotrichum sp.C103 and Penicillium sp.P306 showed broader antifungal spectrum.The strain C103 showed stronger potential with growth inhibition rates higher than 50％ against all of the selected phytopathogens.The minimum inhibition concentrations (MICs) of the EtOAc extract of C103 culture against Botrytis cinerea,Fusarium oxysporum,Myrothecium rorideum was 1.5,0.5 and 0.5 mg/mL,respectively,indicating that endophytic fungi may play an important role in protection of mulberry tree from disease as well as an excellent resource for natural antifungal compounds.