Pseudomonas oleovorans subsp. lubricantis;a newly described bacterium in contaminated metalworking fluids

摘要

Metalworking fluids (MWFs) used as cooling and lubricating agents are highly prone to microbialcontamination. Microbial contamination in MWF is associated with both degradation and health hazards.Among the different microorganisms present in MWFs, species of Pseudomonas act as primarycolonizers. Pseudomonas oleovorans subsp. lubricantis, a newly described bacterium recovered fromused MWF was found to be important to MWF fouling. It was capable of growing in MWF, dropping itspH from 9.2 to 8.0 within 96 hrs indicating its potential as a deteriorgen. However, the actual distributionof this organism in MWF is difficult to study using standard culturing techniques and therefore a realtimePCR (qPCR) assay using TaqMan-MGB technology was developed for its specific identification andestimation. It was also capable of forming biofilm within 72 hrs under static condition. Based on itsbiofouling and biofilm forming capabilities further research is recommended to investigate its impact onMWFs.