Behavior of Cell Cultured on Micro Striped Pattern after Stimulation of Excess Gravity

摘要

The behavior of a single cell cultured on the micro striped pattern has been observed after stimulation of the excess gravity in vitro. The lines of parallel micro quadrangular ridges (0.7 μrn height, 3 μm width, and 3 μm interval) are made on the surface of the plate as the scaffold to make orientation of each cell. Variation is made about the angle between the longitudinal direction of the ridge and the direction of the excess gravity: 0 degree, 45 degrees, and 90 degrees. To apply the excess gravitational force field (50 G, or 100 G) to the cells, the plate was set in the tube in a conventional centrifugal machine placed in an incubator. The contour of each cell at the time lapse images was traced for 24 hours and approximated to ellipse to analyze the angle between the longitudinal axis of the cell and the direction of the excess gravity, after the excess gravitational stimulation for 5 hours. The experiment shows that the longitudinal axis of C2C12 (mouse myoblast cell line) tends to align to the direction of the excess gravity, and returns to the direction of longitudinal direction of micro pattern after stopping of the excess gravity.