Myoblast Behavior around Surface Electrodes in Flow Channel

摘要

The behavior of biological cells around the surface electrode in the flow channel has been observed in vitro. The surface electrodes of titanium (thickness of 200 nm) are made along the edges of the flow channel by the photolithography technique. At the one of the edge, variations are made at the angle of the tip of each surface electrode: 0.26, 0.52, 0.79, or 1.0 rad at triangle or rectangle. The other edge is the reference electrode. One of three modes of cyclic electric stimulations (0.01 ms period) was applied between the surface electrodes: sinusoidal, pulse, or rectangular. Two kinds of cells were used in the test: porcine red blood cells, and C2C12 (mouse myoblast cell line originated with cross-striated muscle of C3H mouse). The suspension of cells was introduced to the flow channel, and the flow rate was controlled by the syringe pump. The experiment shows the following results. The movement of myoblasts changes by the electric stimulation through the surface electrodes during the flow along the channel. The movement depends on the tip angle of the electrode.