Preparation and Characterization of Antherea pernyi silk fibroin scaffold for tissue engineering

摘要

Silk fibroin from Antheraea pernyi (A. pernyi), one of wild silkworms, has different primary structure and mechanical properties from that of domestic silkworm Bombyx mori (B.mori). The primary structure of A. pemyi silk fibroin is consisted of the repeated similar sequences by about 100 times where there are alternative appearance of polyalanine ( PLA) region , (Ala)12-13 and Gly-rich region. Interestingly, the sequence RGD, which is the adhesion motif from fibronectin on extracellular matrix (ECM) proteins, is found in the primary structure of A. pernyi silk fibroin. This implies that A. pernyi fibroin has surface for cell attachment when it is processed as scaffolds in tissue engineering.Therefore, this study tried to find out the potential application of A. pernyi silk fibroin in the field of tissue engineering. The processing ability, biomineralization and cell activity of A. pernyi silk fibroin were investigated. Film was formed by using HFA as solvent. As shown in Figure 1, hydroapatite crystals were formed on A.pernyi silk fibroin through absorbed in 1.5 SBF solution, proving that A.pernyi silk fibroin hasbiomineralized ability. A.pernyi silk fibroin also shows cell adhesion and growth activities in response to NHDF. Therefore, it seems that A.pernyi silk fibroin has potential application as scaffolds in the field of tissue engineering.