The Benefical Effects of Mechanically Stimulating Fibroblasts Seeded on Crimped Electrospun Microfibres

摘要

In 2002, over 200,000 anterior cruciate ligament (ACL) reconstructionsurgerieswerereportedin the USalone,thecostofwhichexceeded five billion dollars . By nature, the ACL has a low self-healing propensity, because it lacks blood supply. The inability of ACL to self-repair has led to the study and design of functional synthetic constructs using tissue engineering principles.Tissueengineeringholdssignificantpromise,but,noengineered ACL tissue has the appropriate extracellular architecture and mechanical properties to allow for successful transplantation. This is due to the lack of scaffolds with adequate structure and mechanical properties capable of supporting ligament development in vitro and remodeling in vivo. Thus, the aim of this study was to fabricate crimped fibres possessing properties similar to nativeACL,with the aimoftestingtheinfluenceofthecrirnpstructreononECMdepostionbyfibrobiasts under mechanical load.