Differentiation of embryonic stem cells into cardiomyocytes on polymeric substrates

摘要

Among the current biomaterial applications, the use of bioabsorbable polymers as cell scaffold has been considered as an alternative therapy in the treatment of injuries and tissue loss, such as during myocardiai infarct Embryonic stem cells (ESC) provide a great therapeutic potential for cardiac tissue. In this study, was assessed the rat ESC interaction with the poly L-co-D,L lactic acid-co-trimethylene carbonate (PLDLA-co-TMC). The characterization of this material by means of SEM, DSC, TGA, GPC, Tensile Test MTT, qPCR and Immunofluorescence techniques. From the obtained results, it was verified that the TMC elements added to PLOLA influenced the material proprieties, providing greater flexibility to the biopolymer and showed weight loss throughout the hydrolytic degradation process. The cell growth and mESC differentiation assays into membranes of PLDLA-co-TMC and Hydrogel, ensure cytocompatibility of these polymers, also evidence that they show no toxicity to the cells which they were grown, allowing adhesion and cell proliferation, as well as the embryonic stem cell differentiation into cardiomyocytes, suggesting that both polymers are acceptable for regenerative cardiac therapy and recommended for use in tissue engineering.