A facile approach to develop multiple trace elements codoped calcium phosphate biomaterials in biomimetic biological medium for osteoporous trauma treatment

摘要

The use of intentional dopants to control the behavior of the biomaterials stands on the heart of many superior biological performances. Trace element (TE)-monodoped calcium phosphates (CaPs) have emerged as a class biomaterials with improved properties that make them potential candidates for bone defect repairs. We show here a more general and reproducible methodology for biomimetic synthesis of a series of binary (i.e. Mg-Zn system), ternary (i.e. Mg-Zn-Sr system), and quaternary (i.e. Mg-Zn- Sr-Si system) TEs-codoped CaP biomaterials in simulated physiological aqueous medium. The morphology observation, structure characterization and composition analysis show that the particle morphology, size, crystallinity and TE contents of the resulting CaP-based nanomaterials can be readily tailored by polypeptide additive, reaction time and TE concentrations. The effects of TEs-codoped CaP on the osteogenic differentiation of ovariectomized rat-derived mesenchymal stem cells (rMSCs) in vitro and efficacy on the bone defect healing in ovariectomized rats were studied. We found that the biomaterials could enhance the osteogenic differentiation of the rMSCs, with up-regulated osteogenic-related gene expression. After implanting the biomaterials into the femoral defect, histology analysis revealed that bone formation was significantly enhanced.