Layer-by-Layer Nanofilm for Simultaneous Stimulation of Bone Cell Attachment and Proliferation and Prevention of Bacterial Colonization

摘要

We developed and engineered multi-drug loaded polypeptide nanofilms using layer-by-layer nanoassembly. The combined incorporation of a cell-adhesive peptide (i.e. RGD), a growth factor (TGF-β1), and a bacterial-killing agent (i.e. gentamicin) on an orthopedic implant has led to enhanced osteoblast adhesion and proliferation as well as effective bacterial killing. The developed multi-drug loaded polypeptide nanofilms may provide a promising means for not only enhancing tissue integration but also simultaneously reducing bacterial infection.