Cloning and Analysis of cDNA Non-coding Sequences of DpXTH1 in Dahlia

摘要

In order to research the structure and function of DpXTHI in dahlia,the first strand cDNA was synthesized by reversed transcription from the RNA of dahlia petals.The 3'-end sequence of 190 bp was amplified by 3'RACE,and the upstream part sequences of start codon have been determined using genome DNA as the template through the high-efficiency TAIL-PCR.The length of 5'-end non-coding sequences was 125 bp.The result preliminarily ascertains the structural features of the cDNA sequences from DpXTH1 of dahlia.